Prevalence and antimicrobial susceptibility
pattern of ESBL producing Gram
Negative Bacilli
Published: April 1, 2011 | DOI: https://doi.org/10.7860/JCDR/2011/.1238
UMADEVI S, KANDHAKUMARI G, JOSEPH N M, KUMAR S, EASOW J M, STEPHEN S, SINGH U K
Dept of Microbiology, Mahatma Gandhi Medical College and
Research Institute, Pillaiyarkuppam, Pondicherry, India
Correspondence
Dr. Sivaraman Umadevi, Associate Professor, Department of
Microbiology, Mahatma Gandhi Medical College and Research
Institute, Pillaiyarkuppam, Pondicherry – 607 402
Email: drumadevis@yahoo.com, Phone: +91 9842173519
The production of extended-spectrum- β lactamases (ESBLs) is an important mechanism for resistance to the third-generation cephalosporins. Awareness and the detection of these enzymes are necessary for optimal patient care.
To determine the prevalence and the antibiotic sensitivity pattern of ESBL producing gram negative bacilli. A prospective study was conducted at a tertiary care teaching hospital
A total of 213 isolates which were recovered between February 2008 and January2009 from various samples were tested for ESBL production by using both the double-disk approximation and the combination disk methods.
Among the 132 Escherichia coli, 54 Klebsiella pneumoniae and 27 Pseudomonas isolates which were tested, 81%, 74%, and 14%, respectively were found to be ESBL producers. The ESBL producing E. coli showed maximum susceptibility to imipenem (100%), followed by piperacillin-tazobactum (84%), amikacin (68%), gentamicin (9%), ciprofloxacin (9%) and amoxicillin-clavulanic acid (7%). Similarly, the ESBL producing K. pneumoniae showed very good susceptibility to imipenem (98%), followed by piperacillin-tazobactum (68%), amikacin (40%), gentamicin (15%), ciprofloxacin (15%) and amoxicillin-clavulanic acid (5%).About 87% and 88% of the ESBL producing E. coli and K. pneumoniae respectively showed multi-drug resistance to amoxicillin- clavulanic acid, gentamicin and ciprofloxacin.
It is essential to report ESBL production along with the routine sensitivity reporting, which will help the clinician in prescribing the proper antibiotics. Piperacillin-tazobactam and imipenem are the most active and reliable agents for the treatment of infections which are caused by ESBL producing organisms.
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